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KMID : 0376219770140010087
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Chonnam Medical Journal
1977 Volume.14 No. 1 p.87 ~ p.96
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Serotyping of Pseudomonas aeruginosa Isolates
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Abstract
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The increasing incidence of infection due to Pseudomonas aeruginosa has led to a requirement in many hospitals for differentiation of strains in order to understand better the associated clinical and epidemiological problems.
Of the many typing schemes available for this nosocomial agent, serotyping seems to offer a more specific and reproducible means of subspeciating this genus.
In the present report, a set of antisera to the seven immunotypes of Fisher has been utilized for differentiation of the 71 strains isolated at Chonnam University Medical School Hospital with the results that serotyping may be a simple, rapid method for differentiation of Pseudomonas species, compared with antibiotic sensitivity, phage -and pyocin typing.
1. Pyocyanine-producing strains among the isolated were only 60%, suggesting that variations are ubiquitous in Pseudomonas colonies.
2. The highest agglutination titer of antiserum was obtained by injecting rabbits with heat-killed, young, and smooth cells as antigen. Acriflavine solution (1%) was applied as a selecting agent of smooth colonies; these smooth cells were cultured for 10 hours, and killed by boiling for one hour at 100°C.
3. Serotyping the 71 isolated according to Fisher´s scheme revealed that immunotype 1 was overwhelming in number (32 strains) while nontypable and the other types scattering aver the rest (2-12 strains).
4. No particular relationship between serotypes, antibiotic sensitivity, and pyocin types was noticed. This was in contrast with the report of Baltimore, et al. where gentamicin sensitivity played an important role when combined with serotyping.
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KEYWORD
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